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Contact Person Mr. ke
shizishan, Wuhan, HUbei
[ Profile] Tetracycline(TCS) ELISA kit is used to detect in Tetracycline, oxytetracycline, chlortetracycline, doxycycline in honey, fish, shrimp , meat , tissue, is widely applied in honey, fish, shrimp , meat industry , ports and government, etc. This kit is Stable , Sample pretreatment and ELISA process is convenient, sensitivity is high , detect limit is 5 ppb. [Principle] This test kit is based on the competitive enzyme immunoassay for the detection of Tetracycline, oxytetracycline, chlortetracycline, doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The TCs in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- TCs antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for ***** (OD) value is determined in **0 nm, (OD) value of the sample has a negative correlation with TCs concentration in the sample the value is compared to the standard curve and the content of the corresponding TCs is subsequently obtained. [Technical data] Detection limit Tissue, milk, chicken, urine, feed ââââââ5ppb Liver, kidneyâââââââââ*0ppb Cross-reaction rate Tetracycline, ââââââââââ**0% Oxytetracyclineââââââââ *7.*9% Chlortetracycline âââââââ *7.*3% Doxycycline âââââââââ *9.*9% Demecloyclineââââââââ *3.*8% Metacyclineâââââââââ 5.*7% Recovery rate Tissue ââââ*0.5ï&frac*2;**3.8% Liver ââââ *5.6ï&frac*2;**2.3% Kidney âââ *2.3ï&frac*2;**8.5% Honey âââ *1.7ï&frac*2;**5.6% Feed âââ *1.7ï&frac*2;**2.3% Milk ââââ *8.5ï&frac*2;**1.5% Egg ââââ *8.1ï&frac*2;**9.8%
